Huilin Li Laboratory

Selected Publications

For a full list of Dr. Li’s publications, please visit his NCBI bibliography.

Zhao P, Zhao C, Chen D, Yun C, Li H*, Bai L*. 2021. Structure and activation mechanism of the hexameric plasma membrane H+-ATPase. Nat Commun 12:6439.
*Co-corresponding authors
Included as an Editor’s Highlight 

Bai L, Jain BK, You Q, Duan HD, Takar M, Graham TR, Li H. 2021. Structural basis of the P4B ATPase lipid flippase activity. Nat Commun 12:5963.

Du M, Yuan Z, Werneburg GT, Henderson NS, Chauhan H, Kovach A, Zhao G, Johl J, Li H, Thanassi DG. 2021. Processive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis. Nat Commun 12(1):5207.

Yu H, Schut GJ, Haja DK, Adams MWW, Li H. 2021. Evolution of complex I-like respiratory complexes. JBC Rev 296:100740.

Yin Y, Feng X, Yu H, Fay A, Kovach A, Glickman MS, Li H. 2021. Structural basis for aggregate dissolution and refolding by the Mycobacterium tuberculosis ClpB-DnaK bi-chaperone systemCell Rep 35(8):109166. 

Zhang H, Hsu HC, Kahne SC, Hara R, Zhan W, Jiang X, Burns-Huang K, Ouellette T, Imaeda T, Okamoto R, Kawasaki M, Michino M, Wong TT, Toita A, Yukawa T, Moraca F, Vendome J, Saha P, Sato K, Aso K, Ginn J, Meinke PT, Foley M, Nathan CF, Darwin KH, Li H, Lin G. 2021. Macrocyclic peptides that selectively inhibit the Mycobacterium tuberculosis proteasomeJ Med Chem 64(9):6262–6272. 

Yin Y, Kovach A, Hsu HC, Darwin KH, Li H. 2021. The mycobacterial proteasomal ATPase Mpa forms a gapped ring to engage the 20S proteasomeJ Biol Chem 100713. 

Bai L, Li H. 2021. Cryo-EM structures of the endoplasmic reticulum membrane complex. FEBS J.

Li H, Zheng F, O’Donnell M. 2021. Water skating: How polymerase sliding clamps move on DNAFEBS J.

Feng X, Schut GJ, Lipscomb GL, Li H, Adams MWW. 2021. Cryoelectron microscopy structure and mechanism of the membrane-associated electron-bifurcating flavoprotein Fix/EtfABCXProc Natl Acad Sci U S A 118(2). 

Bai L, Li H. 2021. Protein N-glycosylation and O-mannosylation are catalyzed by two evolutionarily related GT-C glycosyltransferasesCurr Opin Struct Biol 68:66–73. 

Bohl T, Bai L, Li H. 2021. Recent progress in structural studies on the GT-C superfamily of protein glycosyltransferases. Subcell Biochem96:259–271. 

Li H, Yao NY, O’Donnell ME. 2020. Anatomy of a twin DNA replication factoryBiochem Soc Trans 48(6):2769–2778. 

Zheng F, Georgescu RE, Li H, O’Donnell ME. 2020. Structure of eukaryotic DNA polymerase δ bound to the PCNA clamp while encircling DNAProc Natl Acad Sci U S A 117(48):30344–30353. 

Yu H, Haja DK, Schut GJ, Wu CH, Meng X, Zhao G, Li H, Adams MWW. 2020. Structure of the respiratory MBS complex reveals iron-sulfur cluster catalyzed sulfane sulfur reduction in ancient lifeNat Commun 11(1):5953. 

Yuan Z, Li H. 2020. Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance. Biochem J 477(18):3499–3525. 

Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C. 2020. Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6Proc Natl Acad Sci U S A 117(30):17747–17756. 

Yuan Z, Georgescu R, Schauer GD, O’Donnell ME, Li H. 2020. Structure of the polymerase ε holoenzyme and atomic model of the leading strand replisomeNat Commun 11(1):3156. 

Yuan Z, Georgescu R, Bai L, Zhang D, Li H, O’Donnell ME. 2020. DNA unwinding mechanism of a eukaryotic replicative CMG helicaseNat Commun 11(1):688. 

Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H. 2020. Transport mechanism of P4 ATPase phosphatidylcholine flippases. eLife.  

Bai L, You Q, Feng X, Kovach A, Li H. 2020. Structure of the ER membrane complex, a transmembrane-domain insertase. Nature.

Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O’Donnell ME, Li H. 2019. Ctf4 organizes sister replisomes and Pol α into a replication factoryeLife e47405.

Zhan W, Hsu HC, Morgan T, Oullette T, Burns-Huang K, Hara R, Wright AG, Imaeda T, Okamoto R, Sato K, Michino M, Ramjee M, Aso K, Meinke P, Foley M, Nathan CR, Li H, Lin G. 2019. Selective phenylimidazole-based inhibitors of the Mycobacterium tuberculosis proteasomeJ Med Chem.

Bai L, Kovach A, You Q, Hsu C, Zhao G, Li H. 2019. Autoinhibition and activation mechanisms of the eurkaryotic lipid flippase Drs2p-Cdc50pNat Commun 10(1):4142.

Li H, O’Donnell ME. 2019. DNA replication from two different worlds. Science 363(6429):814–815.

Bai L, Kovach A, You Q, Kenny A, Li H. 2019. Structure of the eukaryotic protein O-mannosyltransferase Pmt1−Pmt2 complex. Nat Struct Mol Biol.

Bai L, Li H. 2019. Cryo-EM is uncovering the mechanism of eukaryotic protein N-glycosylation. FEBS 286(9):1638–1644.

Hu K, Jordan AT, Zhang S, Dhabaria A, Kovach A, Rangel M, Ueberheide B, Li H, Darwin KH. 2019. Characterization of guided entry of tail-anchored proteins 3 homologues in Mycobacterium tuberculosis. J Bacteriol.

Samanovic MI, Hsu HC, Jones MB, Jones V, McNeil MR, Becker SH, Jordan AT, Strnad M, Xu C, Jackson M, Li H, Darwin KH. 2018. Cytokinin signaling in Mycobacterium tuberculosisMBio 19(3).

O’Donnell ME, Li H. 2018. The ring-shaped hexameric helicases that function at DNA replication forks. Nat Struct Mol Biol 25(2):122–130.

Du M, Yaun Z, Yu H, Henderson N, Sarowar S, Zhao G, Werneburg GT, Thanassi DG, Li H. 2018. Handover mechanism of the growing pilus by the bacterial outer-membrane usher FimD. Nature.

Yu H, Wu CH, Schut GJ, Haja DK, Zhao G, Peters JW, Adams MWW, Li H. 2018. Structure of an ancient respiratory system. Cell

Li H, O’Donnell ME. 2018. The eukaryotic CMG helicase at the replication fork: Emerging architecture reveals an unexpected mechanismBioEssays40(3):1700208.    
*Highlighted in Advanced Science News.

Bai L, Wang T, Zhao G, Kovach A, Li H. 2018. The atomic structure of a eukaryotic oligosaccharyltransferase complexNature

Bai L, Yuan Z, Sun J, Georgescu R, O’Donnell ME, Li H. 2017. Architecture of the Saccharomyces cerevisiae replisome. Adv Exp Med Biol 1042:207–228.   

Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li H. 2017. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion modelProc Natl Acad Sci U S A.      

Wu Y, Hu K, Li D, Bai L, Yang S, Jastrab JB, Xiao S, Hu Y, Zhang S, Darwin KH, Wang T, Li H. 2017. Mycobacterium tuberculosis proteasomal ATPase Mpa has a β-grasp domain that hinders docking with the proteasome core protease. Mol Microbiol 105(2):227–241.

Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H. 2017. Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol 24(3):316–324.

Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H, O’Donnell ME. 2017. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A 114(5):E697–E706.

Yu H, Takeuchi H, Takeuchi M, Liu Q, Kantharia J, Haltiwanger RS, Li H. 2016. Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutationsNat Chem Biol 12:735–740.      

Yuan Z, Bai L, Sun J, Georgescu R, O’Donnell ME, Li H. 2016. Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocationNat Struct Mol Biol 23(3):217–224.        

O’Donnell M, Li H. 2016. The eukaryotic replisome goes under the microscopeCurr Biol 26(6):R247-56.       

Bai L, Hu K, Wang T, Jastrab JB, Darwin KH, Li H. 2016. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosisProc Natl Acad Sci U S A 113(14):E1983-92.        

Sun J, Shi Y, Georgescu RE, Yuan Z, Chait BT, Li H, O’Donnell ME. 2015. The architecture of a eukaryotic replisomeNat Struct Mol Biol 22:976–982.

Yu H, Takeuchi M, LeBarron J, Kantharia J, London E, Bakker H, Haltiwanger RS, Li H, Takeuchi H. 2015. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nat Chem Biol 11(11):847–854.

Sun J, Fernandez-Cid A, Riera A, Tognetti S, Yuan Z, Stillman B, Speck C, Li H. 2014. Structural and mechanistic insights into licensing of DNA replicationGenes Dev 28:2291–303.        

Sun J, Evrin C, Samel S, Fernadez-Cid A, Riera A, Kawakami H, Zech J, Stillman B, Speck C, Li H. 2013. Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNANat Struct Mol Biol 20(8):944–951.       

Li H, Stillman B. 2012. The origin recognition complex: a biochemical and structural viewSubcell Biochem 62:37–58.   

Sun J, Li H. 2010. How to operate a cryo-electron microscopeMethods Enzymol 481:231-49.        

Li D, Li Hua, Wang T, Lin G, Li H. 2010. Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S proteasomeEMBO J 29:2037–2047.   

Wang T, Darwin KH, Li H. 2010. Binding-induced folding of prokaryotic ubiquitin-like protein (Pup) targets substrates for proteasomal degradationNat Struct and Mol Biol 17:1352–1357.        

Lin G, Li D, de Carvalho L, Deng H, Tao H, Vogt G, Wu K, Schneider J, Chidawanyika T, Warren JD, Li H, and Nathan C. 2009. Inhibitors selective for mycobacterial versus human proteasomesNature 461:621–626.