Huilin Li Laboratory

Selected Publications

For a full list of Dr. Li’s publications, please visit his NCBI bibliography.

Du M, Yaun Z, Yu H, Henderson N, Sarowar S, Zhao G, Werneburg GT, Thanassi DG, Li H. 2018. Handover mechanism of the growing pilus by the bacterial outer-membrane usher FimD. Nature.

Yu H, Wu CH, Schut GJ, Haja DK, Zhao G, Peters JW, Adams MWW*, Li H*. 2018. Structure of an ancient respiratory system. Cell
*Co-corresponding authors

Li H, O’Donnell ME. 2018. The eukaryotic CMG helicase at the replication fork: Emerging architecture reveals an unexpected mechanismBioEssays40(3):1700208.    
*Highlighted in Advanced Science News.

Bai L, Wang T, Zhao G, Kovach A, Li H. 2018. The atomic structure of a eukaryotic oligosaccharyltransferase complexNature.    

Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li H. 2017. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion modelProc Natl Acad Sci U S A.      

Wu Y, Hu K, Li D, Bai L, Yang S, Jastrab JB, Xiao S, Hu Y, Zhang S, Darwin KH, Wang T, Li H. 2017. Mycobacterium tuberculosis proteasomal ATPase Mpa has a β-grasp domain that hinders docking with the proteasome core protease. Mol Microbiol 105(2):227–241.

Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H. 2017. Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol 24(3):316–324.

Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H*, O’Donnell ME*. 2017. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A 114(5):E697–E706.

Yu H, Takeuchi H, Takeuchi M, Liu Q, Kantharia J, Haltiwanger RS, Li H. 2016. Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutationsNat Chem Biol 12:735–740.      

Yuan Z, Bai L, Sun J, Georgescu R, O’Donnell ME, Li H. 2016. Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocationNat Struct Mol Biol 23(3):217–224.        

O’Donnell M, Li H. 2016. The eukaryotic replisome goes under the microscopeCurr Biol 26(6):R247-56.       

Bai L, Hu K, Wang T, Jastrab JB, Darwin KH, Li H. 2016. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosisProc Natl Acad Sci U S A 113(14):E1983-92.        

Sun J, Shi Y, Georgescu RE, Yuan Z, Chait BT, Li H*, O’Donnell ME*. 2015. The architecture of a eukaryotic replisomeNat Struct Mol Biol 22:976–982.      
* Co-corresponding authors.

Yu H, Takeuchi M, LeBarron J, Kantharia J, London E, Bakker H, Haltiwanger RS, Li H*, Takeuchi H*. 2015. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nat Chem Biol 11(11):847–854.
* Co-corresponding authors.

Sun J, Fernandez-Cid A, Riera A, Tognetti S, Yuan Z, Stillman B, Speck C, Li H. 2014. Structural and mechanistic insights into licensing of DNA replicationGenes Dev 28:2291–303.        

Sun J, Evrin C, Samel S, Fernadez-Cid A, Riera A, Kawakami H, Zech J, Stillman B, Speck C, Li H. 2013. Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNANat Struct Mol Biol 20(8):944–951.       

Li H, Stillman B. 2012. The origin recognition complex: a biochemical and structural viewSubcell Biochem 62:37–58.   

Sun J, Li H. 2010. How to operate a cryo-electron microscopeMethods Enzymol 481:231-49.        

Li D, Li Hua, Wang T, Lin G, Li H. 2010. Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S proteasomeEMBO J 29:2037–2047.   

Wang T, Darwin KH, Li H. 2010. Binding-induced folding of prokaryotic ubiquitin-like protein (Pup) targets substrates for proteasomal degradationNat Struct and Mol Biol 17:1352–1357.        

Lin G, Li D, de Carvalho L, Deng H, Tao H, Vogt G, Wu K, Schneider J, Chidawanyika T, Warren JD, Li H*, and Nathan C*. 2009. Inhibitors selective for mycobacterial versus human proteasomesNature 461:621–626.        
*Co-corresponding authors.

Chen Z, Speck C, Wendel P, Tang C, Stillman B, Li H. 2008. The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiaeProc Natl Acad Sci U S A 105(30):10326–10331.        

Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H. 2008. Fiber formation across the bacterial outer membrane by the chaperone/usher pathwayCell 133(4):640-652.