Huilin Li Laboratory

Selected Publications

For a full list of Dr. Li’s publications, please visit PubMed.

Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li H. 2017. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proc Natl Acad Sci U S A.      Article

Wu Y, Hu K, Li D, Bai L, Yang S, Jastrab JB, Xiao S, Hu Y, Zhang S, Darwin KH, Wang T, Li H. In press. Mycobacterium tuberculosis proteosomal APTase Mpa has a b-grasp domain that hinders docking with the proteasome core protease. Mol Microbiol.       Article

Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen AZ, Barbon M, Rappsilber J*, Stillman B*, Speck C*, Li H*. 2017. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 andCdt1. Nat Struct Mol Biol. doi:10.1038/nsmb.3372        Article

Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H, O’Donnell ME. 2016. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A.       Article

Yu H, Takeuchi H, Takeuchi M, Liu Q, Kantharia J, Haltiwanger RS, Li H. 2016. Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations. Nat Chem Biol 12:735–740.      Article

Yuan Z, Bai L, Sun J, Georgescu R, O’Donnell ME, Li H. 2016. Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation. Nat Struct Mol Biol 23(3):217–224.        PubMed

O’Donnell M, Li H. 2016. The eukaryotic replisome goes under the microscope. Curr Biol 26(6):R247-56.        PubMed

Bai L, Hu K, Wang T, Jastrab JB, Darwin KH, Li H. 2016. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 113(14):E1983-92.        PubMed

Sun J, Shi Y, Georgescu RE, Yuan Z, Chait BT, Li H*, O’Donnell ME*. 2015. The architecture of a eukaryotic replisome. Nat Struct Mol Biol 22:976–982.      PubMed
* Co-corresponding authors.

Yu H, Takeuchi M, LeBarron J, Kantharia J, London E, Bakker H, Haltiwanger RS, Li H*, Takeuchi H*. 2015. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nat Chem Biol 11(11):847–854.        PubMed
* Co-corresponding authors.

Sun J, Fernandez-Cid A, Riera A, Tognetti S, Yuan Z, Stillman B, Speck C, Li H. 2014. Structural and mechanistic insights into licensing of DNA replication. Genes Dev 28:2291–303.        Article

Sun J, Evrin C, Samel S, Fernadez-Cid A, Riera A, Kawakami H, Zech J, Stillman B, Speck C, Li H. 2013. Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA. Nat Struct Mol Biol 20(8):944–51.        PubMed

Li H, Stillman B. 2012. The origin recognition complex: a biochemical and structural view. Subcell Biochem 62:37–58.        PubMed

Sun J, Li H. 2010. How to operate a cryo-electron microscope. Methods Enzymol 481:231-49.        PubMed

Li D, Li Hua, Wang T, Lin G, Li H. 2010. Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S proteasome. EMBO J 29:2037–2047.        PubMed

Wang T, Darwin KH, Li H. 2010. Binding-induced folding of prokaryotic ubiquitin-like protein (Pup) targets substrates for proteasomal degradation. Nat Struct and Mol Biol 17:1352–1357.        PubMed

Lin G, Li D, de Carvalho L, Deng H, Tao H, Vogt G, Wu K, Schneider J, Chidawanyika T, Warren JD, Li H*, and Nathan C*. 2009. Inhibitors selective for Mycobacterial versus human proteasomes. Nature 461:621–626.        Article       PubMed
*Co-corresponding authors.

Chen Z, Speck C, Wendel P, Tang C, Stillman B, Li H. 2008. The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 105(30):10326–10331.        PubMed

Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H. 2008. Fiber formation across the bacterial outer membrane by the chaperone/usher pathway. Cell 133(4):640-652.        PubMed