Huilin Li Laboratory

Selected Publications

For a full list of Dr. Li’s publications, please visit his NCBI bibliography.

Bai L, Kovach A, You Q, Kenny A, Li H. 2019. Structure of the eukaryotic protein O-mannosyltransferase Pmt1−Pmt2 complex. Nat Struct Mol Biol.

Bai L, Li H. 2019. Cryo-EM is uncovering the mechanism of eukaryotic protein N-glycosylation. FEBS 286(9):1638–1644.

Hu K, Jordan AT, Zhang S, Dhabaria A, Kovach A, Rangel M, Ueberheide B, Li H, Darwin KH. 2019. Characterization of guided entry of tail-anchored proteins 3 homologues in Mycobacterium tuberculosis. J Bacteriol.

Du M, Yaun Z, Yu H, Henderson N, Sarowar S, Zhao G, Werneburg GT, Thanassi DG, Li H. 2018. Handover mechanism of the growing pilus by the bacterial outer-membrane usher FimD. Nature.

Yu H, Wu CH, Schut GJ, Haja DK, Zhao G, Peters JW, Adams MWW*, Li H*. 2018. Structure of an ancient respiratory system. Cell
*Co-corresponding authors

Li H, O’Donnell ME. 2018. The eukaryotic CMG helicase at the replication fork: Emerging architecture reveals an unexpected mechanismBioEssays40(3):1700208.    
*Highlighted in Advanced Science News.

Bai L, Wang T, Zhao G, Kovach A, Li H. 2018. The atomic structure of a eukaryotic oligosaccharyltransferase complexNature.    

Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li H. 2017. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion modelProc Natl Acad Sci U S A.      

Wu Y, Hu K, Li D, Bai L, Yang S, Jastrab JB, Xiao S, Hu Y, Zhang S, Darwin KH, Wang T, Li H. 2017. Mycobacterium tuberculosis proteasomal ATPase Mpa has a β-grasp domain that hinders docking with the proteasome core protease. Mol Microbiol 105(2):227–241.

Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H. 2017. Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol 24(3):316–324.

Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H*, O’Donnell ME*. 2017. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A 114(5):E697–E706.

Yu H, Takeuchi H, Takeuchi M, Liu Q, Kantharia J, Haltiwanger RS, Li H. 2016. Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutationsNat Chem Biol 12:735–740.      

Yuan Z, Bai L, Sun J, Georgescu R, O’Donnell ME, Li H. 2016. Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocationNat Struct Mol Biol 23(3):217–224.        

O’Donnell M, Li H. 2016. The eukaryotic replisome goes under the microscopeCurr Biol 26(6):R247-56.       

Bai L, Hu K, Wang T, Jastrab JB, Darwin KH, Li H. 2016. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosisProc Natl Acad Sci U S A 113(14):E1983-92.        

Sun J, Shi Y, Georgescu RE, Yuan Z, Chait BT, Li H*, O’Donnell ME*. 2015. The architecture of a eukaryotic replisomeNat Struct Mol Biol 22:976–982.      
* Co-corresponding authors.

Yu H, Takeuchi M, LeBarron J, Kantharia J, London E, Bakker H, Haltiwanger RS, Li H*, Takeuchi H*. 2015. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nat Chem Biol 11(11):847–854.
* Co-corresponding authors.

Sun J, Fernandez-Cid A, Riera A, Tognetti S, Yuan Z, Stillman B, Speck C, Li H. 2014. Structural and mechanistic insights into licensing of DNA replicationGenes Dev 28:2291–303.        

Sun J, Evrin C, Samel S, Fernadez-Cid A, Riera A, Kawakami H, Zech J, Stillman B, Speck C, Li H. 2013. Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNANat Struct Mol Biol 20(8):944–951.       

Li H, Stillman B. 2012. The origin recognition complex: a biochemical and structural viewSubcell Biochem 62:37–58.   

Sun J, Li H. 2010. How to operate a cryo-electron microscopeMethods Enzymol 481:231-49.        

Li D, Li Hua, Wang T, Lin G, Li H. 2010. Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S proteasomeEMBO J 29:2037–2047.   

Wang T, Darwin KH, Li H. 2010. Binding-induced folding of prokaryotic ubiquitin-like protein (Pup) targets substrates for proteasomal degradationNat Struct and Mol Biol 17:1352–1357.        

Lin G, Li D, de Carvalho L, Deng H, Tao H, Vogt G, Wu K, Schneider J, Chidawanyika T, Warren JD, Li H*, and Nathan C*. 2009. Inhibitors selective for mycobacterial versus human proteasomesNature 461:621–626.        
*Co-corresponding authors.

Chen Z, Speck C, Wendel P, Tang C, Stillman B, Li H. 2008. The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiaeProc Natl Acad Sci U S A 105(30):10326–10331.        

Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H. 2008. Fiber formation across the bacterial outer membrane by the chaperone/usher pathwayCell 133(4):640-652.