Publications

For a full list of Dr. Li’s publications, please visit his NCBI bibliography.

Zhao P, Zhao C, Chen D, Yun C, Li H*, Bai L*. 2021. Structure and activation mechanism of the hexameric plasma membrane H+-ATPase. Nat Commun 12:6439.
*Co-corresponding authors
Included as an Editor’s Highlight 

Bai L, Jain BK, You Q, Duan HD, Takar M, Graham TR, Li H. 2021. Structural basis of the P4B ATPase lipid flippase activity. Nat Commun 12:5963.

Du M, Yuan Z, Werneburg GT, Henderson NS, Chauhan H, Kovach A, Zhao G, Johl J, Li H, Thanassi DG. 2021. Processive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis. Nat Commun 12(1):5207.

Yu H, Schut GJ, Haja DK, Adams MWW, Li H. 2021. Evolution of complex I-like respiratory complexes. JBC Rev 296:100740.

Yin Y, Feng X, Yu H, Fay A, Kovach A, Glickman MS, Li H. 2021. Structural basis for aggregate dissolution and refolding by the Mycobacterium tuberculosis ClpB-DnaK bi-chaperone system. Cell Rep 35(8):109166. 

Zhang H, Hsu HC, Kahne SC, Hara R, Zhan W, Jiang X, Burns-Huang K, Ouellette T, Imaeda T, Okamoto R, Kawasaki M, Michino M, Wong TT, Toita A, Yukawa T, Moraca F, Vendome J, Saha P, Sato K, Aso K, Ginn J, Meinke PT, Foley M, Nathan CF, Darwin KH, Li H, Lin G. 2021. Macrocyclic peptides that selectively inhibit the Mycobacterium tuberculosis proteasome. J Med Chem 64(9):6262–6272. 

Yin Y, Kovach A, Hsu HC, Darwin KH, Li H. 2021. The mycobacterial proteasomal ATPase Mpa forms a gapped ring to engage the 20S proteasome. J Biol Chem 100713. 

Bai L, Li H. 2021. Cryo-EM structures of the endoplasmic reticulum membrane complex. FEBS J.

Li H, Zheng F, O’Donnell M. 2021. Water skating: How polymerase sliding clamps move on DNA. FEBS J.

Feng X, Schut GJ, Lipscomb GL, Li H, Adams MWW. 2021. Cryoelectron microscopy structure and mechanism of the membrane-associated electron-bifurcating flavoprotein Fix/EtfABCX. Proc Natl Acad Sci U S A 118(2). 

Bai L, Li H. 2021. Protein N-glycosylation and O-mannosylation are catalyzed by two evolutionarily related GT-C glycosyltransferases. Curr Opin Struct Biol 68:66–73. 

Bohl T, Bai L, Li H. 2021. Recent progress in structural studies on the GT-C superfamily of protein glycosyltransferases. Subcell Biochem96:259–271. 

Li H, Yao NY, O’Donnell ME. 2020. Anatomy of a twin DNA replication factory. Biochem Soc Trans 48(6):2769–2778. 

Zheng F, Georgescu RE, Li H, O’Donnell ME. 2020. Structure of eukaryotic DNA polymerase δ bound to the PCNA clamp while encircling DNA. Proc Natl Acad Sci U S A 117(48):30344–30353. 

Yu H, Haja DK, Schut GJ, Wu CH, Meng X, Zhao G, Li H, Adams MWW. 2020. Structure of the respiratory MBS complex reveals iron-sulfur cluster catalyzed sulfane sulfur reduction in ancient life. Nat Commun 11(1):5953. 

Yuan Z, Li H. 2020. Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance. Biochem J 477(18):3499–3525. 

Yuan Z, Schneider S, Dodd T, Riera A, Bai L, Yan C, Magdalou I, Ivanov I, Stillman B, Li H, Speck C. 2020. Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6. Proc Natl Acad Sci U S A 117(30):17747–17756. 

Yuan Z, Georgescu R, Schauer GD, O’Donnell ME, Li H. 2020. Structure of the polymerase ε holoenzyme and atomic model of the leading strand replisome. Nat Commun 11(1):3156. 

Yuan Z, Georgescu R, Bai L, Zhang D, Li H, O’Donnell ME. 2020. DNA unwinding mechanism of a eukaryotic replicative CMG helicase. Nat Commun 11(1):688. 

Bai L, You Q, Jain BK, Duan HD, Kovach A, Graham TR, Li H. 2020. Transport mechanism of P4 ATPase phosphatidylcholine flippases. eLife.  

Bai L, You Q, Feng X, Kovach A, Li H. 2020. Structure of the ER membrane complex, a transmembrane-domain insertase. Nature.

Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O’Donnell ME, Li H. 2019. Ctf4 organizes sister replisomes and Pol α into a replication factory. eLife e47405.

Zhan W, Hsu HC, Morgan T, Oullette T, Burns-Huang K, Hara R, Wright AG, Imaeda T, Okamoto R, Sato K, Michino M, Ramjee M, Aso K, Meinke P, Foley M, Nathan CR, Li H, Lin G. 2019. Selective phenylimidazole-based inhibitors of the Mycobacterium tuberculosis proteasome. J Med Chem.

Bai L, Kovach A, You Q, Hsu C, Zhao G, Li H. 2019. Autoinhibition and activation mechanisms of the eurkaryotic lipid flippase Drs2p-Cdc50p. Nat Commun 10(1):4142.

Li H, O’Donnell ME. 2019. DNA replication from two different worlds. Science 363(6429):814–815.

Bai L, Kovach A, You Q, Kenny A, Li H. 2019. Structure of the eukaryotic protein O-mannosyltransferase Pmt1−Pmt2 complex. Nat Struct Mol Biol.

Bai L, Li H. 2019. Cryo-EM is uncovering the mechanism of eukaryotic protein N-glycosylation. FEBS 286(9):1638–1644.

Hu K, Jordan AT, Zhang S, Dhabaria A, Kovach A, Rangel M, Ueberheide B, Li H, Darwin KH. 2019. Characterization of guided entry of tail-anchored proteins 3 homologues in Mycobacterium tuberculosis. J Bacteriol.

Samanovic MI, Hsu HC, Jones MB, Jones V, McNeil MR, Becker SH, Jordan AT, Strnad M, Xu C, Jackson M, Li H, Darwin KH. 2018. Cytokinin signaling in Mycobacterium tuberculosis. MBio 19(3).

O’Donnell ME, Li H. 2018. The ring-shaped hexameric helicases that function at DNA replication forks. Nat Struct Mol Biol 25(2):122–130.

Du M, Yaun Z, Yu H, Henderson N, Sarowar S, Zhao G, Werneburg GT, Thanassi DG, Li H. 2018. Handover mechanism of the growing pilus by the bacterial outer-membrane usher FimD. Nature.

Yu H, Wu CH, Schut GJ, Haja DK, Zhao G, Peters JW, Adams MWW, Li H. 2018. Structure of an ancient respiratory system. Cell. 

Li H, O’Donnell ME. 2018. The eukaryotic CMG helicase at the replication fork: Emerging architecture reveals an unexpected mechanism. BioEssays40(3):1700208.    
*Highlighted in Advanced Science News.

Bai L, Wang T, Zhao G, Kovach A, Li H. 2018. The atomic structure of a eukaryotic oligosaccharyltransferase complex. Nature. 

Bai L, Yuan Z, Sun J, Georgescu R, O’Donnell ME, Li H. 2017. Architecture of the Saccharomyces cerevisiae replisome. Adv Exp Med Biol 1042:207–228.   

Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li H. 2017. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proc Natl Acad Sci U S A.      

Wu Y, Hu K, Li D, Bai L, Yang S, Jastrab JB, Xiao S, Hu Y, Zhang S, Darwin KH, Wang T, Li H. 2017. Mycobacterium tuberculosis proteasomal ATPase Mpa has a β-grasp domain that hinders docking with the proteasome core protease. Mol Microbiol 105(2):227–241.

Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H. 2017. Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol 24(3):316–324.

Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H, O’Donnell ME. 2017. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A 114(5):E697–E706.

Yu H, Takeuchi H, Takeuchi M, Liu Q, Kantharia J, Haltiwanger RS, Li H. 2016. Structural analysis of Notch-regulating Rumi reveals basis for pathogenic mutations. Nat Chem Biol 12:735–740.      

Yuan Z, Bai L, Sun J, Georgescu R, O’Donnell ME, Li H. 2016. Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation. Nat Struct Mol Biol 23(3):217–224.        

O’Donnell M, Li H. 2016. The eukaryotic replisome goes under the microscope. Curr Biol 26(6):R247-56.       

Bai L, Hu K, Wang T, Jastrab JB, Darwin KH, Li H. 2016. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 113(14):E1983-92.        

Sun J, Shi Y, Georgescu RE, Yuan Z, Chait BT, Li H, O’Donnell ME. 2015. The architecture of a eukaryotic replisome. Nat Struct Mol Biol 22:976–982.

Yu H, Takeuchi M, LeBarron J, Kantharia J, London E, Bakker H, Haltiwanger RS, Li H, Takeuchi H. 2015. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nat Chem Biol 11(11):847–854.

Sun J, Fernandez-Cid A, Riera A, Tognetti S, Yuan Z, Stillman B, Speck C, Li H. 2014. Structural and mechanistic insights into licensing of DNA replication. Genes Dev 28:2291–303.        

Sun J, Evrin C, Samel S, Fernadez-Cid A, Riera A, Kawakami H, Zech J, Stillman B, Speck C, Li H. 2013. Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA. Nat Struct Mol Biol 20(8):944–951.       

Li H, Stillman B. 2012. The origin recognition complex: a biochemical and structural view. Subcell Biochem 62:37–58.   

Sun J, Li H. 2010. How to operate a cryo-electron microscope. Methods Enzymol 481:231-49.        

Li D, Li Hua, Wang T, Lin G, Li H. 2010. Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S proteasome. EMBO J 29:2037–2047.   

Wang T, Darwin KH, Li H. 2010. Binding-induced folding of prokaryotic ubiquitin-like protein (Pup) targets substrates for proteasomal degradation. Nat Struct and Mol Biol 17:1352–1357.        

Lin G, Li D, de Carvalho L, Deng H, Tao H, Vogt G, Wu K, Schneider J, Chidawanyika T, Warren JD, Li H, and Nathan C. 2009. Inhibitors selective for mycobacterial versus human proteasomes. Nature 461:621–626.